Author: restaumatic

18 jan In the present data, an effective durum grain (Triticum turgidum L

var. durum) population out-of 1step 36 recombinant inbred outlines (RILs) try sex when you look at the soil significantly less than regulated conditions to determine the fresh chromosome nations which might be involved in the power over their sources and you will take tissues.

dos.step 1. Hereditary Product

The latest RIL people away from 136 F6 traces that was found in the present data was created from a cross between your Italian durum grain cv. “Simeto” (Capeiti/Valnova) and you will a good cultivar of T. dicoccum labeled as “Molise Colli” which was picked inside framework regarding a local population regarding T. dicoccum (in the Regional Department for Advancement and you can Innovation out of Farming from the fresh new Molise Part (Agenzia Regionale per lo Sviluppo e l'Innovazione dell'Agricoltura della Regione Molise)).

dos.dos. Bush Increases and you will Crushed Sampling

The RIL population and the parents were grown in plastic cylinders containing a soil mixture (soil: sand, 50 : 50; v/v). Before the pot experiments, soil with a history of exposure to annual cereal species was collected (in ) from the experimental farming station of the Cereal Research Centre in Foggia (Italy; cuatro1°28? N, 15°34? E; 76 m a.s.l.). The samples were collected from the upper 30 cm of the soil profile and air dried for 1 week. They were then thoroughly mixed, passed through a 2 mm sieve (to remove gravel fragments), cleaned of plant debris, and stored in a cold room (4°C) until further use. This soil was an unsterilized loam soil (USDA classification system) with the following characteristics: 21% clay, 43% silt, 36% sand, pH 8 (in H₂O), 15 mg/kg available phosphorous (Olsen method), 800 mg/kg exchangeable potassium (NH₄Ac), and 21 g/kg organic matter (Walkey-Black method).